What is the emission spectrum of PerCP-Cy5 5?
PerCP (Peridinin chlorophyll)-Cy5. 5 is a fluorescent compound with an excitation peak at 482 nm and an emission peak at 695 nm.
What is the excitation emission wavelength of Cy5 5?
Cy5. 5 (Cyanine-5.5) is a fluorescent compound with an excitation peak at 683 nm and an emission peak at 703 nm.
What is the difference between PerCP and PerCP-Cy5 5?
532 nm 695/40 PerCP-Cy™5.5 (Exmax482 nm/Emmax695 nm) is a tandem conjugate that combines PerCP with the cyanine dye Cy5. 5. PerCP-Cy5. 5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers.
What is the alternative to PerCP-Cy5 5?
BD Horizon Brilliant™ Blue 700 (BB700) (Exmax 485 nm/Emmax 693 nm) is a dye that was exclusively developed by BD Life Sciences as brighter alternative to PerCP-Cy5. 5. This dye also has less cross-laser excitation off the 405-nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.
What color is the emission of PerCP?
Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter.
What color is PE Cy5 5?
PE-Cyanine®5.5 (PE-Cy5. 5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5.
Are Cy5 and Cy5 5 the same?
Cy5 and Cy5. 5 are both cyanine dyes with several differences between them. Used in Fluorescence Resonance Energy Transfer (FRET), Fluorescence Polarization (FP), and Time Resolved- Fluorescence Resonance Energy Transfer (TR-FRET).
What is the excitation coefficient of Cy5?
The molar extinction coefficient (ε) for Cy5 (Cyanine-5) is 250000 cm–1M–1 in Ethanol. To request additional information and datasets for Cy5 (Cyanine-5), please contact [email protected]. Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH2O or PBS.
What is the emission of Cy3 and Cy5?
Cy3-Cy5 Tandem is a fluorescent compound with an excitation peak at 650 nm and an emission peak at 668 nm.
Which laser is PerCP-Cy5 5?
PerCP-Cy5. 5 can be excited with a standard 488 nm laser and emits in the far red at 695 nm. There is no spectral overlap into the PE channel; thus, it is well suited for multicolor flow cytometry experiments with FITC, PE, and other fluorochrome conjugates.
What is the wavelength of PerCP?
PerCP is a water soluble carotenoid pigment found in photosynthetic dinoflagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm.
What is the alternative to Cy5 5?
TYE 705. A bright fluorescent dye that can be used in microarray applications. TYE 705 can be used as a direct substitute for Cy5. 5®.
What is the wavelength of Cy5 5?
Cy5. 5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 678 nm and an emission peak at 695 nm. Spectrally its similar to Alexa Fluor™ 680.
What is the excitation emission of Cy5 channel?
Cy5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 649 nm and an emission peak at 667 nm.
Can you see Cy5 by eye?
Although the human eye can detect light in the emission range of Cy5, its sensitivity in the long-red/ far-red range is low (Lennie et al. 1993). Therefore, Cy5 has generally been regarded as unsuitable for di- rect visualization and observation (Brelje et al.
Is PerCP a tandem dye?
PerCP-eFluor 710—a tandem dye excited with the blue (488 nm) laser; two to three-fold brighter than PerCP-Cy5. 5. eFluor 660—an organic dye excited with the red (633 nm) laser; an alternative dye option to APC or Alexa Fluor® 647.
Why is FITC green?
FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.
What is the full name of PerCP?
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has a high quantum efficiency and a large Stokes shift. It is well excited at 488 nm with its maximum emission peak at 677nm.
What is similar to PerCP Cy5 5?
BB700 was developed as a brighter alternative to PerCP-Cy5. 5, making it better suited for resolving dim populations (Figure 5). Figure 5c shows how a dimmer dye such as PerCP-Cy5.
Is Cy5 red or green?
APC-Cy5. 5. It exhibits a strong fluorescence in the far red region of the spectrum. Like all tandems conjugates, APC-Cy5.
What filter to use with Cy5?
This Iridian single band filter set consists of the 628-40 EX, 692-40 EM and 660 DM filters. It is optimised for the cyanine dye Cy5. The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third-party manufacturers.
What is PerCP Cy5 5?
Peridinin chlorophyll protein-Cyanine5. 5 (PerCP-Cyanine5. 5) is a photostable tandem dye excited from the 488 nm laser. It is stable in fixative, and stained cells can be stored overnight.
What is the emission maximum for Cy5?
PE-Cy5 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-phycoerythrin (PE) donor that has excitation maxima (Ex-Max) of 496 nm and 566 nm and an acceptor dye, Cy5, with an emission maximum (Em-Max) at 670 nm.
Is Cy5 far-red?
Invitrogen Cyanine5 (Cy5) dye is a bright, far-red-fluorescent dye with excitation designed for use with the 633 nm or 647 nm laser lines.
What is the excitation emission spectra of PE Cy5?
Fluorescence emission spectra of R-Phycoerythrin and PE-Cy5. Excitation is at 488 nm and the emission spectra are normalized to the absorbance of both dyes at 488 nm. PE-Cy5 has a peak emission spectrum at 669 nm where there is little overlap with the R-PE spectrum. This reduces compensation required to less than 10%.
What is the wavelength of PerCP excitation emission?
PerCP (Peridinin chlorophyll) is a fluorescent compound with an excitation peak at 482 nm and an emission peak at 678 nm.
What is Cy5 used for?
Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions.
What is the emission of Cy3 and Cy5?
Cy3-Cy5 Tandem is a fluorescent compound with an excitation peak at 650 nm and an emission peak at 668 nm.
What is the wavelength of PerCP?
PerCP is a water soluble carotenoid pigment found in photosynthetic dinoflagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm.
What is the wavelength of the Cy5 filter?
The visible light absorption maximum of Cy5 is 649 nanometers and the emission maximum occurs at 670 nanometers.
What is the color of Cy5 fluorophore?
Invitrogen Cyanine5 (Cy5) dye is a bright, far-red-fluorescent dye with excitation designed for use with the 633 nm or 647 nm laser lines.
What spectral overlap does percp-cy5 have?
How does a PerCP molecule work?
What is the spectra of PerCP (peridinin chlorophyll)-Cy5.5?
Why is percp-cy5.5 used?
Okay, so you’re interested in Percp Cy5.5 excitation and emission, huh? That’s a pretty specific topic, but I’m happy to break it down for you. Let’s dive into the world of fluorescence and see what makes Percp Cy5.5 so special.
What is Percp Cy5.5?
Percp Cy5.5 is a type of fluorescent dye. Now, you might be wondering what a fluorescent dye is. Think of it like a tiny lightbulb that only turns on when you shine a specific kind of light on it.
Excitation and Emission: The Basics
When we talk about fluorescence, we talk about two key concepts: excitation and emission.
Excitation: This is the process of giving the dye energy by shining a specific wavelength of light on it. Think of it as charging up the tiny lightbulb.
Emission: Once the dye has absorbed the energy, it releases that energy as light at a different, longer wavelength. This is like the lightbulb turning on and shining.
Percp Cy5.5: A Bright Star
Percp Cy5.5 is particularly interesting because it’s known for its bright fluorescence. This means it emits a lot of light when excited.
Why is Percp Cy5.5 Important?
Percp Cy5.5 is commonly used in various applications, including:
Flow cytometry: Imagine analyzing cells, but instead of just looking at their size and shape, you can also see what proteins they express. Percp Cy5.5 helps us tag and identify these proteins!
Immunofluorescence microscopy: This technique allows us to see different parts of cells by using antibodies that are attached to fluorescent dyes, like Percp Cy5.5.
Biotechnology research: Percp Cy5.5 is valuable in studying DNA and protein interactions.
Understanding the Numbers: Excitation and Emission Wavelengths
Now, let’s talk numbers. Percp Cy5.5 has a specific excitation wavelength and a specific emission wavelength. This means it absorbs light at a particular wavelength and emits light at another wavelength.
Excitation Wavelength: Percp Cy5.5 is typically excited at a wavelength around 649 nm (nanometers).
Emission Wavelength: It emits light at a wavelength around 670 nm.
Think of it like this:
* You shine a red light (649 nm) on Percp Cy5.5.
* The dye absorbs that red light.
* It then releases a slightly longer wavelength of light (670 nm), which appears as a red-orange color.
How Does Percp Cy5.5 Work in Flow Cytometry?
Flow cytometry is a powerful technique that uses lasers to excite fluorescent dyes attached to cells. Here’s how Percp Cy5.5 fits in:
* Cell Staining:Percp Cy5.5 is used to label cells with specific antibodies. These antibodies bind to specific proteins on the cell surface.
* Excitation: A laser excites the Percp Cy5.5 dye, causing it to emit fluorescence.
* Detection: The emitted fluorescence is detected by specialized sensors that measure the intensity of the signal.
* Data Analysis: Based on the intensity and wavelength of the fluorescence, we can identify different cell populations and the proteins they express.
Percp Cy5.5: A Versatile Tool
Percp Cy5.5 is a powerful tool for researchers and scientists. Its bright fluorescence, specific excitation and emission wavelengths, and compatibility with different technologies make it indispensable for a wide range of applications.
FAQs
1. What is the difference between Percp Cy5.5 and Percp Cy5?
While both dyes are similar, they differ in their spectral properties. Percp Cy5.5 has a slightly red-shifted emission spectrum compared to Percp Cy5. This means it emits light at a slightly longer wavelength.
2. Can Percp Cy5.5 be used with other fluorescent dyes?
Absolutely! Percp Cy5.5 can be used in combination with other fluorescent dyes, as long as they have distinct excitation and emission spectra. This allows researchers to label multiple targets simultaneously.
3. What are some common applications of Percp Cy5.5?
Percp Cy5.5 is widely used in:
Immunofluorescence microscopy
Flow cytometry
Cell sorting
Biotechnology research
DNA sequencing
4. Is Percp Cy5.5 toxic?
Percp Cy5.5 is generally considered safe for use in laboratory settings. However, like all chemicals, it’s important to handle it with proper safety precautions.
5. How can I learn more about Percp Cy5.5?
You can find more information about Percp Cy5.5 in scientific journals, online databases, and manufacturer websites. There are also many tutorials and resources available online.
Remember: While Percp Cy5.5 is a great tool for research, it’s important to understand its specific properties and potential limitations before using it in your experiments.
See more here: What Is The Excitation Emission Wavelength Of Cy5 5? | Percp Cy5 5 Excitation Emission
PerCP-Cy5.5 Dye Profile – FluoroFinder
The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an FluoroFinder
Spectrum [PerCP (Peridinin chlorophyll)-Cy5.5] – AAT Bioquest
PerCP (Peridinin chlorophyll)-Cy5.5 is a fluorescent compound with an excitation peak at 482 nm and an emission peak at 695 nm. Other spectra of interest include: PE-iFluor™ AAT Bioquest
BD PerCP-Cy5.5 and PerCP – BD Biosciences
PerCP-Cy5.5 can be excited with a standard 488 nm laser and emits in the far red at 695 nm. There is no spectral overlap into the PE channel; thus, it is well suited for multicolor BD Biosciences
PerCP-Cyanine5.5 dye | Thermo Fisher Scientific – US
Peridinin chlorophyll protein-Cyanine5.5 (PerCP-Cyanine5.5) is a photostable tandem dye excited from the 488 nm laser. It is stable in fixative, and stained cells can be stored overnight. Thermo Fisher Scientific
PE-Cy5.5 Dye Profile – FluoroFinder
PE-Cyanine®5.5 (PE-Cy5.5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5.5. The donor molecule, PE can be excited by the 488-nm FluoroFinder
PerCP-Cyanine5.5 dye | Thermo Fisher Scientific – IN
Peridinin chlorophyll protein-Cyanine5.5 (PerCP-Cyanine5.5) is a photostable tandem dye excited from the 488 nm laser. It is stable in fixative, and stained cells can be stored Thermo Fisher Scientific
PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control – BD Biosciences
This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission BD Biosciences
PerCP-Cy™5.5 Streptavidin – BD Biosciences
PerCP-Cy5.5 is a tandem fluorochrome composed of peridinin chlorophyll protein (PerCP), which is excited by the 488-nm line of an Argon ion laser and serves as the energy BD Biosciences
PerCP-Cy5.5 Tandem Catalog number: 2650 Unit size: 1 mg
Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of aatbio.com
Fluorochrome/Laser Reference Poster – Carver College of Medicine
PerCP-Cy™5.5 (Ex max 482 nm/Em max 695 nm) is a tandem conjugate that combines PerCP with the cyanine dye Cy5.5. PerCP-Cy5.5 is not subject to photobeaching like Carver College of Medicine
See more new information: charoenmotorcycles.com
Running A Basic 2 Color Flow Cytometry Experiment In Bd Facs Diva
Multicolor Flow Cytometry – Understanding The Basics [Webinar]
Fluorescence And Compensation In Flow Cytometry
Nanolive Imaging: Prea Labeled With Mitonir, Imaged With Nanolive + Cy5 Excitation Light
Fluorescence-Based Monitoring Of Pad4 Activity: Pro-Fluorescence Substrate Analog L Protocol Preview
Compensation In Flow Cytometry Webinar
Nir To Vis – Nir Up – Conversion And X-Ray Excited Emission Of Er Doped High Z Biocl
Labelling Cells With Fluorophores.Avi
Facs Diva Tutorial
Link to this article: percp cy5 5 excitation emission.
See more articles in the same category here: https://charoenmotorcycles.com/how